Super-resolution imaging technology amps up dendrite spine investigations

Published on : Sep 30, 2019

Albany, New York, September 30, 2019: Super-resolution imaging technology in life sciences is perceived as minimally invasive technique that acquires data rapidly and provides new advances such as 3D analysis in in various area including microbiology, genetics and neurosciences. Super resolution imaging techniques help in early diagnosis of medical conditions such as cancer.

Super-resolution imaging technology can be broadly segmented into two groups that are techniques imaging molecules with illumination patterns and techniques imaging single molecule. The former group can be segregated into Stimulated emission depletion (STED) and Structured illumination microscopy (SIM) techniques, and latter into Stochastic Optical Reconstruction Microscopy (STORM) and Photo activated localization microscopy (PALM).

LIMON presides diagnosis

A broader spectrum of information can be obtained with combined application of super resolution imaging technologies. Light Microscopical Nano sizing (LIMON) microscopy, a combination of two techniques, namely, Spectral Precision Distance/Position Determination Microscopy (SPDM) and Spatially Modulated Illumination (SMI) microscopy, performs dual color reconstruction to obtain additional information of 3D spatial arrangement of cellular structures.

Advancements in super-resolution imaging technologies is led by development of associated tools and technologies such as fluorophores. Effective selection of fluorophores from the existing verities, for super-resolution imaging technique can help to derive a robust result. Ultraviolet rays exhibit the tendency to damage living cells with long exposure and selection of suitable fluorophore can avoid this phenomena.

Neuroscience is highly benefitted by super-resolution imaging technology as it visualizes any change in dendrite spine present in living cell which are very small and essential for neuronal function. Imaging techniques help in studies to understand situational dynamics of synapsis. Super resolution imaging technology has enabled imaging of Dendrite spines like never before. Investigation of cytoskeleton with nanometer thick filament is possible with super-resolution imaging technology.

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